Content Determination of Diammonium Glycyrrhizinate Preparation by HPLC

OBJECTIVE To establish an HPLC method for the content determination and related substance of diammonium glycyrrhizinate preparation. METHODS Agilent Extend-C₁₈ column(4.6 mm×250 mm, 5 μm)was used with the mobile phase acetonitrile-0.01 mol·L^-1 phosphate solution(38∶62), the flow rate was 1.0 mL·min^-1, determination wavelength at 252 nm. RESULTS The linear range of the method was 12.5-1 250 μg·mL^-1(r=0.999 9), the limit of detection was 5.2 ng, the recovery of diammonium glycyrrhizinate was >99.4%. CONCLUSION The method is convenient, accurate and can be used for quality control of diammonium glycyrrhizinate preparation.