Effect of Antler Ethanol Extract on Promoting the Decomposition of Adipose Tissue in Vitro

OBJECTIVE To study the effect of various parts of ethanol extract of pilose antler(AEPA) on the decomposition of adipose tissue in vitro and the active factors in decomposing of fat. METHODS Cervus Nippon antler was taken as material. Antler wax slice, powder slice and blood slice was taken and dissolved into 75% ethanol, respectively, and extracted thrice. Then the antler ethanol extract of various parts were centrifuged and combined the supernatant, last freeze-drying and studied on each part of ethanol extract to promote the role of adipose tissue breakdown in vitro. The content of hypoxanthine in each sample of velvet antler was determined by HPLC. RESULTS Hypoxanthine and lipolysis were in linear relationship, speculated the factor that activity of promoting fat decomposition might be hypoxanthine. The hypoxanthine, the ethanol extract of wax slice, powder slice and blood slice could facilitate the decomposition of rat fat regardless, when adrenalin hydrochloride was added, the decomposition rates were 472.00%, 183.20%, 145.56% and 115.84%, respectively; when adrenalin hydrochloride was not added, the decomposition rates were 220.00%, 104.74%, 112.80%, respectively, the ethanol extract of blood slice had no such effect. The contents of hypoxanthine in wax slice, power slice and blood slice were 0.386, 0.268, 0.162 mg·g^-1, respectively. CONCLUSION The wax slice promote lipolysis in vitro is the strongest, powder slice is followed and blood slice is weakest, hypoxanthine may be active factor in promoting lipolysis.