Determination of Bakuchiol in Serum, Liver and Kidney of Rats

OBJECTIVE To establish a quantitaive determination method of bakuchiol in serum, liver and kidney of rats by HPLC. METHODS The HPLC method was used with the Agilent HC-C₁₈ and the mobile phase was 0.1% formic acid-acetonitrile (65∶35) 0-10 min, B 35%→45%, 10-25 min, B 80% in the flow rate of 1 mL·min^?1. The detection wavelength was set at 295 nm(chloramphenicol) in 0-10 min and 260 nm(bakuchiol) in 10-25 min with column temperature of 30 ℃. RESULTS The bakuchiol had a good linear between 0.1-10.0 mg·L^?1 in serum, liver and kidney of rats. CONCLUSION This method can determine the content of bakuchiol in serum, liver and kidney of rats.