Simultaneous Determination of Nucleoside Contents in Compound Shougong Powder by UPLC

OBJECTIVE To establish a UPLC method for the determination of adenine and adenosine in Compound Shougong powder. METHODS The determination was performed on Acquity BEH C₁₈(2.1 mm×100 mm, 1.7 μm) with mobile phase consisted of methabol-water(gradient elution) at a flow rate of 0.25 mL·min^-1. Column temperature was 26 ℃. The UV detection wavelength was set at 254 nm. RESULTS The calibration curves were linear in the range of 0.040 9-0.511 5 mg·mL^-1(r=0.999 9) for adenine, 0.057 8-0.722 0 mg·mL^-1(r=0.999 6) for adenosine. The average recoveries were 97.7%, 97.7% and RSDs were 0.96%, 1.18%, respectively. CONCLUSION The determination method is fast, reliable, accurate, and can be used for quality control of Compound Shougong powder.