Study on Enrichment of Ginsenoside Rb₁ in Shenxue Capsules by Macroporous Adsorptive Resin

OBJECTIVE To study the technological parameters of the purification process of ginsenoside Rb₁ in Shenxue capsules by macroporous adsorption resin. METHODS Measured the content of ginsenoside Rb₁ by HP1100, the HPLC conditions were ZORBAX SB-C₁₈ column(4.6 mm×250 mm, 5 μm); the detector wavelength was 203 nm; the mobile phase was acetonitrile-water(30∶70). The type of resin was selected by the resin adsorption, elution volume ratio and the content of ginsenoside Rb₁. At the same time, wash solvent was selected and the L₉(3⁴) orthogonal experiment was carried out to study the elution conditions, in order to determine the purification technological parameters of macroporous adsorption resin. RESULTS The linear of ginsenoside Rb₁ had a good relationship from 0.752 μg to 9.4 μg. Determine the washing solvent solution was 0.5 mol·L^-1 NaOH and the optimal elution conditions were: extraction after standing for 12 h in column, with 15 times 60% ethanol, and the fiowrate was 1.0 mL·min^-1. CONCLUSION The method was simple and exclusive, which can be available to enrich ginsenoside Rb₁ in Shenxue capsules.