Progress of Different Elution Modes of Counter-current Chromatography

OBJECTIVE To review the recent progress on various kinds of elution modes emerged during the modern counter-current chromatographic techniques in detail. METHODS Almost all the related literatures about studying on different kinds of elution modes for counter-current chromatography were summarized, in which its applications, advantages and disadvantages of each elution mode were in introduced. RESULTS Totally seven types of elution modes that frequently used in the modern counter-current chromatography were described, including gradient elution mode, dual-mode elution, multiple-dual mode, closed-loop recycling mode, elution-extrusion mode, elution-back extrusion mode and pH-zone-refining mode. CONCLUSION Each of elution mode for counter-current chromatography owns its distinctive characteristic and should be selected in light of analytes be separated by this technique, in order to achieve better separations.