Determination of Vitamin A in the Form of Microcapsule in Functional Food by HPLC

OBJECTIVE To develop an HPLC method for determination of vitamin A in the form of microcapsule in functional food. METHODS Microcapsule structure was disrupted by suitable ammonia solution and vitamin A was extracted by organic solutions. The chromatographic separation was achieved on an Agilent ZORBAX SB-C₁₈ column(250 mm×4.6 mm, 5 μm) with methanol as the mobile phase. The detection wavelength was at 325 nm, flow rate 1.0 mL·min^-1 and the column temperature 35 ℃. RESULTS The linear range of vitamin A was 1.41-56.4 μg·mL^-1(r=1.000). The average recovery of vitamin A was 97.5%(RSD=1.9%). CONCLUSION The established method was simple, accurate and with good reproducibility, suitable for content determination of vitamin A in functional food.