Determination of Scopoletin, Baicalin, Baicalein and Farrerol in Qinbaohong Zhike Capsule by HPLC

OBJECTIVE To establish a method for determining the content of scopoletin, baicalin, baicalein and farrerol in Qinbaohong Zhike capsule. METHODS An Eclipse XDB-C₁₈ column (4.6 mm×250 mm, 5 μm) was used with methanol (A)-1% acetic acid (B) as the mobile phase at the flow rate of 0.8 mL·min^-1, and the UV-detection wavelength was set at 295 nm with the column temperature of 30 ℃. RESULTS Scopoletin showed a good linear relationship at the range of 1.27-124.70 μg (r=0.999 9), the average recovery was 98.3% with RSD of 1.1%. Baicalin showed a good linear relationship at the range of 8.07-538.20 μg(r=1.000 0), the average recovery was 98.0% with RSD of 1.2%. Baicalein showed a good linear relationship at the range of 1.12-111.60 μg(r=1.000 0), the average recovery was 98.5% with RSD of 0.9%. Farrerol showed a good linear relationship at the range of 1.06-106.40 μg(r=1.000 0), the average recovery was 99.0% with RSD of 1.4%. CONCLUSION This method is simple, accurate and reproducible. It can be reliable for the quality control of Qinbaohong Zhike Capsule.