Study on HPLC Fingerprint of Four Kinds of Drugs of Jingzhi Guanxin Keli Fufang

OBJECTIVE To establish the chromatographic fingerprint of Jingzhi Guanxin Keli Fufang by HPLC. METHODS HPLC was performed on Jingzhi Guanxin Keli Fufang using Agilent 1100 DAD-HPLC. Inertsil ODS-3 column (4.6 mm×250 mm, 5 μm) was used; the mobile phase was acetonitrile-0.1% phosphoric acid for gradient elution; the flow rate was 1 mL·min^-1; the detection wavelength was 270 nm; the column temperature was 30 ℃, and inject volume was 10 μL. RESULTS Twenty-two HPLC common peaks were acquired in the 10 batches of four kinds of drugs of Jingzhi Guanxin Keli Fufang, and their similarities were >0.90. Good precision, stability and repetition were showed. CONCLUSION A reliable method is provided for the quality identification of Jingzhi Guanxin Keli Fufang. And the chemical components of different germplasms of Jingzhi Guanxin Keli Fufang are different from each other; selection of germplasm is important in quality control of Jingzhi Guanxin Keli Fufang.