Preparation and Its Application of Sulfamethoxazole Molecularly Imprinted Polymer Sensor

OBJECTIVE To electroploymerize a molecularly imprinted polymer(MIP) film for sulfamethoxazole detection on a glassy carbon electrode by the use of pyrrole monomer. METHODS The effects of sulfamethoxazole concentration, polypyrrole concentration, number of scan cycle and scan rate on the MIP film were investigated respectively. The effects of pH value and acetonitrile-water ratio of detection liquid on response current were also explored. The imprinted film was characterised by electrochemical impedance spectroscopy(EIS) and cyclic voltammetry (CV). RESULTS The results indicated the DPV peak current showed a linear dependence on the sulfamethoxazole concentration in the ranges of 2.50×10^-5-7.50×10^-4 mol·L^-1 and 7.50×10^-4-2.00×10^-3 mol·L^-1 of sulfamethoxazole(each correlation coefficient of 0.995 8 and 0.996 7, respectively) with the detection limit (S/N=3) of 2.80×10^-6 mmol·L^-1, under the optimal experimental conditions. CONCLUSION This MIP film electrode also displayed good selectivity and reproducibility and stability. The prepared sensor was applied to the determination of sulfamethoxazle in Paediatric Compound Sulfamethoxazole Tablet samples with recovery ranging from 94.2% to 105.0%.