Long-term Effects of Kudiezi Injection on Contralateral Testis with Unilateral Testicular Torsion/Detorsion in Prepubertal Rats

OBJECTIVE To observe long-term effects of Kudiezi injection on contralateral testis with unilateral testicular torsion/detorsion in prepubertal rats, and investigate the protective effect of Kudiezi injection on it. METHODS Thirty-two four-week-old healthy male SD rats were divided into four groups randomly: sham operation group, model group, once injection of Kudiezi group and continuous injection of Kudiezi group(n=8). Animals were submitted to unilateral 720°testicular torsion, then detorsion were done in 2 h. The rats were killed after 8 weeks of surgery and excised their right testes for testicular coefficient calculation, the measurement of T-AOC, SOD, NOS activity and MDA content in the testis and tissue histopathological examinations were drawed. RESULTS Compared with the sham operation group, the model group exhibited a remarkable decrease in SOD, T-AOC, NOS activity and an obvious increase in MDA content(P<0.05). Compared with the model group, the continuous injection group exhibited a significant increase in SOD, T-AOC, NOS activity, and an obvious decrease in MDA content(P<0.05). Interstitial edema and degeneration of seminiferous tubules were observed in the model group. Testicular tissues in injection of Kudiezi group showed an improved histological morphology. CONCLUSION The contralateral testis of prepubertal rats can be damaged after unilateral testicular torsion/detorsion. Kudiezi injection has the long-term protective effect in prepubertal rat testicular injury after torsion/detorsion through effective scavenging oxygen free radicals, inhibition of lipid peroxidation, and the effect of the continuous injection group is better than the once injection group.