Rapid Determination of Cefalexin Granules by NIR

OBJECTIVE To develop a NIR method for rapid determination of Cefalexin granules. METHODS The NIR spectra of 187 batches of Cefalexin granules from different pharmaceutical manufacturers were collected, 94 spectra of them were used as the calibration set and the rest 93 spectra as the validation set. The quantitative model was set up and applied to predict the validation set and detect the samples. RESULTS For the quantitative determination, the concentration range of cephalexin was 0.042 0-0.138 0 mg·mg^-1. The determination coefficients (R²) of the cross validation was 98.84, the true mean predictive error of root meansquare error of cross validation (RMSECV) was 0.003. The mean prediction error of root mean square error of prediction (RESEP) was 0.003, the correlation coefficient of the true value and prediction value was 0.995 0. CONCLUSION The method is simple, rapid and accurate, and can be applied to drug rapid determination.