RRLC-QTOF Applied to the Impurities Profile of Lacosamide

OBJECTIVE To study the impurities profile of lacosamide with RRLC-QTOF method. METHODS The high performance liquid chromatography and quadrupole-flight time tandem mass spectrum (RRLC-QTOF) were used to analyze lacosamide. The column was XDB-C₁₈(150 mm×4.6 mm, 5 μm). Acetonitrile and ammonium acetate buffer (pH4.5) were used as mobile phase with gradient elution. The total flow was 1 mL·min^-1 and 0.3 mL·min^-1 flowing into mass spectrum by a joint. RESULTS Combined the high resolution mass and MS² fragment ions, the possible structure of four impurities in lacosamide were infered. CONCLUSION It is good reference for the research of the impurities profile in lacosamide.