Determination of Mogroside V in Fufang Luohanguo Zhike Granule by HPLC-ELSD

OBJECTIVE To establish an HPLC-ELSD method for the determination of morgtoside V in Fufang Luohanguo Zhike granule. METHODS AlltimaTM-C₁₈ column(4.6 mm×250 mm, 5 μm), mobile phase: acetonitrile-water(23∶77), column temperature: 30 ℃. ELSD condition: temperature of drift tube: 105 ℃, flow rate of gas: 2.0 mL·min^-1. RESULTS Morgtoside V was linear in the range of 1.608-9.648 μg, with regression equation of Y=1.634 3X+12.591(r=0.999 7), the average recovery was 101.36%(RSD=1.29%). CONCLUSION The established method is accurate, specific and reliable for the determination of morgtoside V in Fufang Luohanguo Zhike granule.