Monitoring the Safety of Carbamazepine by Loop Mediated Isothermal Amplification

OBJECTIVE To establish a technology platform for testing the marker gene HLA-B*1502 of severe epispasis induced by carbamazepine using the loop-mediated isothermal amplification(LAMP). METHODS The marker gene HLA-B* 1502 was tested by LAMP method and the SSP-PCR method, respectively. And the accuracy of the LAMP method was verified. RESULTS The genotype results of control group and drug eruption group tested with LAMP method were consistent with those using SSP-PCR method. CONCLUSION LAMP method is simple and quick to be operated. It’s reliable and accurate, and can be widely applied in clinic.