Separation of Bortezomib and Its Optical Isomers on Amylose Chiral Stationary Phase by NP-HPLC

OBJECTIVE To develop a normal phase HPLC method for the separation of bortezomib and its optical isomers. METHODS The chiral separation of bortezomib and its optical isomers on amylase tris (3,5-dimethylphenylcarbamate) chiral stationary phase was carried out. The effectes of different proportions of n-hexane/isopropanol/ethanol in mobile phase were investigated, and the flow rate of mobile phase and column temperature were also optimized. RESULTS The mobile phase of the established method was n-hexane-isopropanol-ethanol(86∶6∶8), with the flow rate of 0.6 mL·min^-1. The column temperature was set at 30 ℃. CONCLUSION The method is simple, rapid and can be used for analysis of bortezomib and its optical isomers.