Determination of Cynanchogenin and Caudatin in Cynanchum Otophyllum Scheid by HPLC under Double UV Wavelengths

OBJECTIVE To estabish an HPLC method under double UV wavelengths for determination of cynanchogenin and caudatin in Cynanchum otophyllum Scheid. METHODS ZORBAX SB-C18 column (4.6 mm×150 mm, 5 μm) was used and the temperature of column was 40 ℃. The mobile phase consisted of methanol-acetonitrile with gradient elution. The flow rate was 1.0 mL·min^-1. The detection wavelength was set at 220 and 260 nm. RESULTS The cynanchogenin and caudatin had a good linearity relationship in 2 μg·mL^-1, with r=1. The average recoveries were 100.1% and 97.9% with RSD of 1.34% and 1.37%(n=6). CONCLUSION The method is simple, rapid, accurate and provides the reference for the quality identification of Cynanchum otophyllum Scheid and its preparation.