Quantitative Determination of Three Local Anesthetics in Delay Drugs by UPLC-MS/MSHUANG Zhaohui1, CAI Dandan2, CHEN Zhongyi1, ZHUO Kaihua1 (1 Ningbo Institute for Drug Control, Ningbo 315048, China;2 Ningbo Drug Research Center, Ningbo 315010, China;)

ABSTRACT: OBJECTIVE To establish a method for the quantitative determination of 3 local anesthetics in delay drugs by UPLC-MS/MS. METHODS Chromatography was carried on a Waters BEH C18 column(50 mm×2.1 mm, 1.7 μm) with the column temperature at 35 ℃, using 0.02 mol?L-1 ammonium acetate water solution(containing 0.1% acetic acid) -acetonitril (72:28) as mobile phase at a flow rate of 0.2 mL?min-1. Determination was performed by multiple reaction monitoring mode using electrospray ionization in positive ion mode. RESULTS Three chemical medicines had good linear relationships in 20 μg?L-1-200 μg?L-1 (r=0.9988-0.9998). average recovery were 98.2%-101.2%.The limits of quantitation and detection were 0.5 μg?L-1 and 0.1 μg?L-1, respectively. CONCLUSION The method is rapid, sensitive and accurate, and could be an appropriate technique to detect the local anesthetics in delay drugs.