Supercritical Fluid CO₂ Extraction Technology of Flavonoids from Callicarpa Kwangtungensis Chun.

OBJECTIVE To study the technology of supercritical fluid CO₂ extraction (SFE CO2) on flavonoids from Callicarpa kwangtungensis Chun. METHODS　The effects of pressure, temperature, time and volume of entrainer on flavonoids extracts were studied by orthogonal test. The optimum condition for SFE CO₂ was determined. RESULTS　The optimum extraction conditions were as follows: pressure 40.0 MPa, temperature 40 ℃, time 1.0 h and absolute ethyl alcohol entrainer, content 4.5 mL·g^-1 materials. The yield of flavonoids was 6.27%, and the content was 65.21%. CONCLUSION The optimum extraction has high extraction ratio of flavonoids. The optimum extraction is reasonable and practicable1.