Establishment of Immunology Rapid Test Method of HupA in Rare Source Plant Huperzia serrata

: Objective Preparation and characterization of monoclonal antibodies against Huperzine A, and establishing a determination method for HupA in Huperzia serrata．Methods：BALB/c mice were immunized by the conjugation of HupA–BSA successfully, and monoclonal antibodies were generated by hydridoma technique. Then hybridomas were cloned by the limiting dilution method and four hybridomas producing McAb reactive to HupA named 3E02, 4B01, 5D03 and 4C04 were obtained, a enzyme-linked immunosorbent assay for HupA was established successfully. Result: McAb isotype were IgG2b, IgG2b, IgG1 and IgG1.The ELISA standard curve was Y=0.363X-0.2377, r=0.9920, and the range of linearity was 5-1000 ng/mL. The recovery was 96.6-103.8%, RDS 0.68%(n=6). Conclusion: The ELISA method provided a rapid,sensitive and accurate procedure for the determination of HupA in Huperzia serrata samples．