Determination of Dexamethasone Palmitate in Electrospun Nanofiber Membrane for Ocular Application

OBJECTIVE To establish an HPLC method for determination of dexamethasone palmitate in nanofiber membrane for ocular application. METHODS The chromatographic analysis was performed on a Diamosil C₁₈ column (150 mm×4.6 mm, 5 μm) at 40 ℃. The mobile phase was 100% methanol. Flow rate was 1.0 mL·min^-1 and detection wavelength was 239 nm, injection volume was 20 μL. RESULTS Good liner relationship was obtained in the range of 0.1-5 μg·mL^-1(r=0.999 5). Both intra- and inter-day precision RSD were <2%(n=5). The average recovery rate was 99.89% with RSD of 1.15% (n=15). CONCLUSION The established method is sensitive, reliable and suitable for assay of dexamethasone palmitate in nanofiber membrane for ocular application.