Study on Separation and Purification of Syringin, Aleutheroside E and Isofraxidin from Acanthopanax Senticosus by Macroporous Resin

OBJECTIVE To study the separation and purification technology of syringin, eleutheroside E and isofraxidin from Acanthopanax senticosus by macroporous resin. METHODS The adsorption and desorption rate of four types of macroporous resins was compared, so as to determine the proper kind of resin and the best condition for purifying. RESULTS The adsorption and isolation effect of AB-8 resin was much better. The optimal conditions were as follow: the concentration of sample solution was 0.5 g·mL^-1, the adsorption flow rate was 2 BV·h^-1, the maximum loading amount was 5 BV, the eluent was 30% ethanol, the dosage was 11 BV and the elution flow rate was 1 BV·h^-1. Under the optimal conditions, the content of syringin, eleutheroside E and isofraxidin in purified powder was 3.13%, 1.82% and 0.42%, respectively. CONCLUSION AB-8 is an ideal resin with the best enrichment for separating and purifying syringin, eleutheroside E and isofraxidin simultanously.