The Fingerprint Research of Engelhardia Roxburghiana Leaves from Origination in Guangxi

OBJECTIVE To study the RP-HPLC fingerprints of 11 batches of Engelhardia Roxburghiana leaves from different regions of Guangxi province for their quality control. METHODS The fingerprint spectrum was determined by HPLC-UV. The HPLC separation was achieved on a reverse phase Diamonsil? C18 (2) column (4.6 mm i.d.× 250 mm, 5 μm ) with a C18 guard column (4.6 mm i.d. ×15 mm, 5 μm) maintained at 30 ℃. The mobile phase was acetonitrile-water containing 0.05% phosphoric acid (19:81, v/v). The flow rate was set at 1.0 mL?min-1. The detection wave length was 240 nm. The injection volume was 10 μL. The analyzing time was 60 min. Similarity evaluation system for chromatographic fingerprint of TCM published by Chinese Pharmacopoeia Commission (Version: 2004A) was used in data analysis. RESULTS A sensitive, reproducible and selective detection method and a rapid, stable and simple extraction procedure were developed and validated. A reference fingerprint spectrum was established and 11 co-possessing peaks were selected as fingerprints. The similarity between the reference fingerprint spectrum and each fingerprint spectrum from the eleven samples was analyzed and all the similarity values were not less than 0.95. CONCLUSION The method for fingerprint spectrum establishment was scientific and reasonable and can be used for quality control of Engelhardia Roxburghiana leaves from origination in Guangxi.