Growth Inhibition and Apoptosis Induction Effects of Genistein on K562 Cells

OBJECTIVE To study the growth inhibition and apoptosis induction effects of genistein on human leukemia cell. METHODS Growth inhibition effect of genistein on K562 cells was investigated by MTT colorimetry. Morphologic variations of genistein on K562 cells was detected by Wright-Gimesa. Genistein induced apoptosis of K562 cells was detected by flow cytometry. RESULTS Genistein could powerfully inhibit cell growth of K562 cells line. Morphology showed high genistein concentration cell nuclear apoptosis bodies appeared. Cell cycle analysis showed that the highly concertration genistein induced the growth inhibition and apoptosis on K562 cells(P<0.05). CONCLUSION Genistein make a much sensitive reaction on K562 cells. K562 cells can be significantly inhibited G2/M phase arrest, genistein induces apoptosis on K562 cells.