Determination of Gitoxin and Digitoxin in Digaoxin Tablets by HPLC-MS/MS
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Graphical Abstract
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Abstract
OBJECTIVE To establish an HPLC-MS/MS method for determining the contents of gitoxin and digitoxin in Digaoxin tablets. METHODS The condition of detection were: Agilent C18(150 mm×4.6mm, 5 μm), the mobile phase consisted of acetonitrile and water(40∶60), the detection wavelength was triple quadrupled tandem mass spectrometry with ESI source in the negative ion mode. Quantitation was performed using multiple reaction monitoring (MRM). RESULTS The calibration curves were linear in the range of 0.025 65-2.565 μg·mL-1 for gitoxin(r=0.999 3); the calibration curves were linear in the range of 0.025 95-2.596 μg·mL-1 for digitoxin(r=0.999 8). The average recovery of gitoxin was 99.7%(RSD=2.2%); the average recovery of digitoxin was 100.9%(RSD=2.3%). CONCLUSION The method is simple, accurate, reliable and can be used for the quality control of gitoxin and digitoxin in Digaoxin tablets.
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