Determination of Free Sucrose in Sucrose Stearate by HPLC-ELSD

OBJECTIVE An HPLC-ELSD method was established for the determination of the free sucrose in sucrose stearate. METHODS A NH2 column was used with mobile phase A of 10 μg?mL?1 ammonium acetate in acetonitrile and mobile phase B of 10 μg?mL?1 ammonium acetate in tetrahydrofuran - water (90:10) by gradient elution. Evaporative light scattering detector was used. RESULTS The calibration curve of sucrose was linear in the concentration range of 0.25 – 2.5 mg?mL?1. The average recovery was 100.5%, with RSD of 0.8%. CONCLUSION The method was sensitive, selective, and could be used for the determination of free sucrose in sucrose stearate.