Cytotoxicity of 2-Dodecenoic Acid on Human H4 Cells

OBJECTIVE To study 2-dodecenoic acid toxicity mechanism on human H4 cells. METHODS Toxic effects of 2-dodecenoic acid on human H4 cells were detected with methods of MTT and flow cytometry. RESULTS Given the human H4 cells 0.3, 0.9, 2.7 mg·L^-1 2-dodecenoic acid for 48 h, human H4 cells proliferation inhibition rates were 41.64%, 70.97%, 81.52%, respectively, and the 50% inhibitory concentration(IC50) was 0.40 mg·L^-1, compared with the control group, the difference was statistically significant(P<0.01); apoptosis rates were 10.16%, 18.22%, 24.06%, respectively. CONCLUSION 2-Dodecenoic acid on human H4 cells has significant toxic effects, its mechanism may be related to the destruction of the cell mitochondrial structure and inhibition of cell division.