UPLC Method for Rapid Detection of Lianhuaqingwen Capsule Ingredients in the Water Extract Content

ABSTRACT: OBJECTIVE To develop an fast determination method for Lianhua Qingwen Capsule Water extraction process intermediates. METHODS UPLC method was used to determine the content of neochlorogenic acid, chlorogenic acid, liquiritin, 3,4 - caffeoylquinic acid, 4,5 - caffeoylquinic acid, glycyrrhizic acid. RESULTS The quantitative method is simple, rapid, accurate. Neochlorogenic acid in 4.32~21.6ng (r=0.9998 ), Chlorogenic acid in 6.82~34.08ng (r=0.9998 ), Liquiritin in 2.49~9.33ng (r=0.9998 ), 3,4- two coffee caffeoylquinic acid in 16.35~81.76ng (r=0.9997 ), 4,5- two coffee caffeoylquinic acid in 14.42~72.08ng (r=0.9997 ), Glycyrrhizic acid in 4.19~20.96ng (r=0.9996 ) had good linearity. The average recovery rates ( n=6 ) were 97.10, 96.66, 97.34, 98.98, 98.97, 97.80%, RSD were 0.82, 1.30, 1.31, 1.17, 1.63, 1.73% ( n=6 ). CONCLUSION This method can effectively control the Lianhua Qingwen capsule Water extract quality.