Chemical Constituents of Wuling Fermentative Powder

OBJECTIVE To study the chemical constituents of Wuling fermentative powder. METHODS Four components were extracted, separated and purified from the parts of petroleum and acetic ether by the means of silica gel column chromatography and preparative HPLC methods, and identified by NMR data. RESULTS The four compounds were elucidated as: 5-hydroxymellein(1), 5-carboxylmellein(2), genistein(3) and 5-methylmellein(4). CONCLUSION The later three components are obtained for the first time from Wuling Fermentative powder. 1, 2 and 4 are the characteristic components in Wuling Fermentative powder.