To study the changes of quantity of effective constituent in different Medicinal properties medicines after compatibility with Gypsum fibrosum

ABSTRACT: OBJECTIVE：By study the changes of quantity of effective constituent after compatibility of Cimicifuga foetida、 Cinnamomum cassia Presl、 Glycyrrhiza radix et rhizome、Scutellariae radix respectively with Gypsum fibrosum by HPLC to research the four gas theory of traditional chinese medicine .Methods: Agilent Eclipse XDB-C18(150 mm×4.6 mm, 5 μm) was adopted; the mobile phase was Acetonitrile- water of 0.1% phosphoric acid(13:87) the detection wavelength was 254 nm for Caffeic acid Ferulic acid Isoferulic acid; the mobile phase was Acetonitrile- water of 0.1% phosphoric acid(32:68) the detection wavelength was 290 nm for Cinnamic aldehyde Cinnamic acid;the mobile phase was methanol-H2O- phosphoric acid(47:53:0.2) the detection wavelength was 277 nm for Baicalin; Agilent Eclipse XDB-C18(250 mm×4.6 mm, 5 μm) was adopted; the mobile phase was 0.1% phosphoric acid solution(A)- Acetonitrile(B) with gradient elution the detection wavelength was 237 nm for Liquiritin Glycyrrhizic acid Results: The quantity of chemical componts in compatibility of Cim;icifuga foetida、Scutellariae radix with Gypsum fibrosum increased; The quantity of chemical componts in compatibility of Cinnamomum cassia Presl Glycyrrhiza radix et rhizome with Gypsum fibrosum reduced. Conclusion: Gypsum fibrosum can add the chemical components quantity of The cold drugs but reduce the chemical components quantity of The warm drugs