Optimization of Extraction Technology for Polysaccharides in Inonotus Obliquus

OBJECTIVE To optimize the extraction process for polysaccharides in Inonotus obliquus. METHODS The content of Inonotus obliquus polysaccharides was used as index based on the one-factor tests, and the method of response surface method with 4 factors and 3 levels was adopted. The content of heavy metal in the extraction was detected. RESULTS The optimum conditions of Inonotus obliquus polysaccharides extraction as follows: ethanol concentration was 30%, extraction temperature was 95 ℃, time of extraction was 2.5 h and the ratio of material to solvent was 30∶1. Response surface analysis predicted that the production of crude polysaccharide was 33.6% and the content of polysaccharide was 6.177%, respectively. By using verification test, Inonotus obliquus polysaccharides reached 5.993% without significant difference compared with the prediction. And the level of heavy metal significantly decreased. CONCLUSION The optimized extraction process can obtain high extraction rate, low level of heavy metal content, and can be used for extraction of Inonotus obliquus polysaccharides.