Determination of Polyphyllin Ⅰ, Ⅱ, Ⅵ and Ⅶ in Gongxuening Capsule by HPLC

OBJECTIVE　To establish an HPLC method for determination of polyphyllin Ⅰ, Ⅱ, Ⅵ and Ⅶ in Gongxuening capsule. METHODS Phenomenex Luna C₁₈ column (4.6 mm×250 mm, 5 μm) was used. Mobile phase was acetonitrile(A)- water(B) with gradient elute. The flow rate was 1.0 mL·min^-1, the column temperature was 25 ℃, the detection wavelength was 203 nm. RESULTS The content of polyphyllin Ⅰ, Ⅱ, Ⅵ and Ⅶ had a good linear relationship in the range of 2.024- 14.168, 2.010-14.07, 2.016-14.112 and 2.032-14.224 μg·mL^-1, and r was 0.999 8, 0.999 7, 0.999 8 and 0.999 8, respectively. The average recoveries of them were 99.0%(RSD=0.29%), 99.4%(RSD=1.13%), 99.4%(RSD=0.58%), 100.3(RSD=0.95%), respectively. CONCLUSION The methods is simple and quick, which has good precision and stability, the result is reliable and can be used for quality control of Gongxuening capsule.