Determination of the Content of Effective Component of Wild and Cultivated Products of Hedyotis Diffusa

OBJECTIVE To determine the content of the active component of wild and cultivated products of Hedyotis diffusa, provide a basis for its quality standards and standardized cultivation. METHODS The content of oleanolic acid and ursolic acid in Hedyotis diffusa was determined by HPLC, and the content of total flavonoids was determined by UV. RESULTS The content of three kinds of active component were significantly different in different areas of wild products, and the content of oleanolic acid was 0.328 1-1.191 7 mg·g^-1; the content of ursolic acid was 1.686 5-2.641 0 mg·g^-1; the content of total flavonoids was 0.921 9%-1.611 7%. The content in the three kinds of Guangdong and Guangxi cultivated products were higher, there was no significantly difference between them, and kept certain stability. CONCLUSION Application of the home-grown product instead of wild products of Hedyotis diffusa, can ensure the effectiveness and stability of the quality.