Determination of Chlorogenic Acid, Rutin, and Luteolin Glycosides in Mulberry Leaves by HPLC

OBJECTIVE To determine the content of chlorogenic acid, rutin and luteolin glycosides in mulberry leaves by HPLC. METHODS The sample was extracted by 70% ethanol and separated on a Phenomenex Luna column(4.6 mm×250 mm, 5 μm). 0.1% Phosphoric acid and acetonitrile was used as mobile phase with gradient elution at flow rate of 1.0 mL·min^-1 The detection wavelength was set at 348 nm. RESULTS The linear ranges of chlorogenic acid, rutin and luteolin glycosides were 3.048-152.4, 1.059-52.96 and 1.437-71.85 μg·mL^-1. And the average recoveries were 100.4%, 98.3% and 98.7% with the RSD of 1.7%, 2.0%, 1.9%(n=9), respectively. CONCLUSION This method is simple, rapid and with good repeatability, and can be used for the quality control of mulberry leaves.