Determination of Sodium Bisulfite in Azasetron Hydrochloride and Sodium Chlodide Injection by Ion Chromatography

OBJECTIE To establish an ion chromatography method for rapid and precise determination of the content of antioxidant sodium bisulfite in azasetron hydrochloride and sodium chlodide injection. METHODS The AS-11 anion analytical column(4.6 mm×250 mm, 5 μm) was adopted to separate the samples with the eluent of 20 mmol·L^-1 potassium hydroxide. The flow rate was 1.0 mL·min^-1, and the suppressed conductivity detector was adopted. Samples were diluted with 0.4% formaldehyde solution followed by direct injection. RESULTS This method exhibited excellent regression(r=0.9997) in the range of 4.07-30.51 μg·mL^-1 for NaHSO₃ and regression(r=0.999 8) in the range of 1.59-159.0 μg·mL^-1 for Na₂SO₄, the average recovery (n=9) was 98.82%, 98.24%, respectively for NaHSO₃ and Na₂SO₄. CONCLUSION The method is simple, accurate, stable and precise, and can be userd for the determination of sodium bisulfite in azasetron hydrochloride and sodium chlodide injection.