Study on the Derivatization conditions for Formaldehyde and Acetaldehyde

OBJECTIVE To establish the derivatization conditions for controlling the contents of formaldehyde and acetaldehyde by HPLC method at the same time. METHODS After derivatization of which formaldehyde and acetaldehyde was mixed thoroughly with the reagent solution (2,4-DNPH) and heated at 60 ℃ in a water bath for 60 min, the test was performed on Agilent TC-C₁₈ (250 mm×4.6 mm, 5 μm) column with the mobile phase of water-acetonitrile (45∶55) at a flow rate of 1.0 mL·min^-1. The detection wavelength was set at 350 nm and the column temperature was 40 ℃. The injection volume was 20 μL. RESULTS The resolution of the mixture solution of derivatization was 7.2. The linear ranger of formaldehyde was 0.028~9.23 μg·mL^-1 (r=0.999 4), the average recovery (n=9) was 102.6% (RSD=0.9%), LQDformaldehyde was 3.7×10^-6 μg, LODformaldehyde was 1.8×10^-6μg; The linear ranger of acetaldehyde was 0.063~20.9 μg·mL^-1(r=0.999 5), the average recovery (n=9) was 102.0% (RSD=1.2%), LOQacetaldehyde was 1.1×10^-4 μg, LODacetaldehyde was 5.5×10^-5 μg. CONCLUSION The method is accurate and sensitive. The study may provide a basis for the safety monitoring of formaldehyde and acetaldehyde.