Determination of 2,5’-Dibromo-4,5,2’-trihydroxydiphenylmethanone and Its Related Substances by HPLC

OBJECTIVE To establish an HPLC method for the determination of 2,5’-dibromo-4,5,2’-trihydroxydiphenyl- methanone(LM49) and its related substances. METHODS HPLC method was carried on a column of Diamonsil C₁₈ column (250 mm × 4.6 mm, 5 μm) with the mobile phase of methanol-acetonitrile-water (adjusted to pH 3.0 with phosphoric acid) (70∶10∶20) at the flow rate of 0.8 mL·min^-1 and the detection wavelength was set at 261 nm. The column temperature was 25 ℃. RESULTS LM49 was completely separated from the impurities. A good linearity(r=0.999 9) of LM49 was obtained at the concentration range of 1.0-100 mg·L^-1. The minimal detection limit was 0.02 mg·L^-1. The average recovery of method was 99.8%(RSD=0.88%). CONCLUSION The analytical method is simple, rapid, sensitive and accurate for the determination of LM49 and its related substances.