Simultaneous Determination of Five Flavonoids in Callicarpa Kwangtungensis Chun. by Microemulsion Liquid Chromatography

OBJECTIVE To establish an efficient microemulsion liquid chromatography(MELC) method and validate for simultaneous determination of five flavonoids (5,4′-dihydroxy-3,7,3′-trimethoxyflavone, rhamnatin, kumatakenin, ermanine and velutin) in Callicarpa kwangtungensis Chun.. METHODS The MELC separation was performed on a Hypersil BDS C_l8 column(4.6 mm×150 mm, 5 μm) with the mixture of 2.2% SDS-1.5% octane-7.75% n-butanol-0.5% triethylamine-88% water, the pH was adjusted to 3.7 with phosphoric acid as the mobile phase. The flow rate was set at 0.8 mL·min^-1 and the eluent was detected at 350 nm for the five flavonoids．RESULTS The calibration curves of the five flavonoids were linear (r≥0.999 5) over the concentration range of 1.520-72.25 μg·mL^-1. The mean recoveries were 99.6%-100.5%. CONCLUSION The results indicate that the optimized method is successfully applied to the analysis of five important flavonoids in Callicarpa kwangtungensis Chun..