Rapid TLC Identification and Simultaneous Determination of Stilbene Glucoside and Salrianolic Acid B for Guidan Shaji Capsules

OBJECTIVE To rapidly control the quality of dietary supplement by multiple indicators, the method of quality control for Guidan Shaji capsules were studied. METHODS Polygoni Multiflori Radix, Salviae Miltiorrhizae Radix et Rhizoma, Hippophae Fructus, Angelicae Sinensis Radix and Armeniacae Semen Amarum were identified by TLC at 2 thin-layer chromatographic plates. The contents of stilbene glucoside and salvianolic acid B in the samples were determined using a mixture of methanol-acetonitrile-formic acid-H2O(24∶9∶1∶66) as the mobile phase by HPLC. RESULTS The methodological study showed that a good linear correlation existed in the range 0.042 3-0.846 μg of stilbene glucoside (r=0.999 5) and 0.118-2.36 μg for salvianolic acid B(r=0.999 6), respectively. Their average recoveries were 99.04% and 98.47%, respectively, and the RSD(n=9) were 0.66% and 0.83%, respectively. The negative sample did not interfere TLC identification. CONCLUSION This method of quality control is simple, rapid and usable which can be used for quality control of Guidan Shaji capsules.