Simultaneous Determination of Six Ingredients in Xingjiang Ramulus Mori by HPLC

OBJECTIVE To establish an HPLC method for simultaneous determination of chlorogenic acid, rutin, isoquercitrin, astragalin, resveratrol and morin in Ramulus Mori. METHODS The separation was carried out on a PMC-pack ODS(4.6 mm×250 mm, 5 μm) column with 0.2% phosphoric acid-0.2% phosphoric acid in acetonitrile as a mobile phase at a flow rate of 0.6 mL·min^-1 with a gradient program. The detection wavelength was 360 nm. RESULTS Six ingredients were completely separated within 45 min. The average recoveries were between 97.7% and 100.7%(RSD≤2.0%). CONCLUSION The method is simple, rapid, reproducibility, accurate and reliable which can be used as the reference for the quality control of Ramulus Mori.