Simultaneous Determination of Bergapten and Ferulic Acid in Seselopsis by HPLC

OBJECTIVE To simultaneous determine the content of ferulic acid and bergapten in Seselopsis. METHODS The chromatographic separation was performed on a PhenomeneX Gemini-NX C18 110A (250 mm×4.60 mm, 5μm). The mobile phase was consisted of methanol-0.05% phosphoric acid with gradient elute, the flow rate was 1.0 mL·min^-1. The detection wavelength was 322 nm and the column temperature was 30 ℃. RESULTS Good linear relationship between the peak area and content of ferulic acid and bergapten was achieved when the amount was within 0.020 1-0.603 μg, 0.020 3-0.609 μg; the recoveries were 96.8% and 98.7%; RSD were 2.0% and 2.9%. CONCLUSION This is the first time to simultaneous determine ferulic acid and bergapten in Seselopsis. The method is accurate, reproducible and suitable to be used for quality assessment of Seselopsis.