Study on Rat Intestinal Absorption of Naringenin and Hesperetin in Situ

OBJECTIVE To investigate the absorption behavior of naringenin and hesperetin, and the effect of P-glycoprotein (P-gp) on these two components. METHODS The rat intestinal perfusion model was employed. The concentrations of naringenin, hesperetin in the intestinal perfusate samples were determined by ultra performance liquid chromatography(UPLC), and the effective permeability coefficient (P_eff*) and 10 cm of bowel absorption percentage were calculated. RESULTS The Peff* values of naringenin (20 μmol·L^-1) in duodenum, jejunum, ileum and colon were 2.77±0.43, 2.39±0.30, 1.90±0.53, 3.15±0.30, respectively, while the P_eff* values of hesperetin (20 μmol·L^-1) in the four intestines were 2.66±0.43, 2.45±0.12, 2.09±0.14, 3.33±0.18, respeactively. After adding the P-gp inhibitor verapamil, the absorption of these two ingredients in the four intestines were significantly increased(P<0.05). CONCLUSION These results further confirme that naringenin and hesperetin are both absorbed well, and they both can be effluxed by P-gp in the intestinal mucosa.