Determination of Related Substances in Trityl Losartan by HPLC

OBJECTIVE To establish an HPLC method for determination of related substances in trityl losartan. METHODS Lichrospher RP-select B 60A column (250 mm×4.0 mm, 5 μm)was used, the gradient mobile phase consisting of 0.01 mol·L^-1 sodium dihydrogen phosphate(pH=2.5)-acetonitrile with a flow rate of 1.5 mL·min^-1 was used. The column temperature was kept at 35 ℃ and the detection wavelength was set at 230 nm. RESULTS Trityl losartan and related substances could be well isolated. CONCLUSION The method is simple, rapid, accurate and suitable for determinating the related substances in trityl losartan.