Study on the Chemical Constituents of the Aerial Parts of Cynanchum Chinense R. Br.

OBJECTIVE To study the chemical constituents of the aerial parts of Cynanchum chinense R. Br. METHODS The compounds were extracted and purified by AB-8 macroporous resins, silica gel column chromatography, LX2000 resin and Sephadex LH-20 columns. Their structures were elucidated on the basis of physico-chemical properties and NMR spectral analysis. RESULTS Nine compounds were obtained from 70% aqueous ethanol extract of Cynanchum chinense and elucidated as n-hexadecanioc acid (Ⅰ), salicylic acid (Ⅱ), β-sitosterol (Ⅲ), β-sitosterolglucoside (Ⅳ), apigenin (Ⅴ), kaemperol (Ⅵ), kaemperol-3-O-β-D-glunoside (Ⅶ), tricin (Ⅷ), and tricin-7-O-β-D-glucuronide (Ⅸ). CONCLUSION Compounds Ⅴ, Ⅷ and Ⅸ were obtained from this genus, and compounds Ⅰ, Ⅱand Ⅴ-Ⅸ were isolated from this plant for the first time.