Content Determination of Aucubin in Huai-Rehmannia Radix by HPLC

OBJECTIVE To analyse the aucubin content in Huai-Rehmannia Radix from different varieties and habitats and to preliminarily evaluate the germplasm resources. METHODS HPLC method was used with the column packed with Dikma Diamonsil C₁₈(4.6 mm×250 mm, 0.5 μm), the mobile phase was a mixture of methanol and water in a ratio of 5.5∶94.5, the detection wavelength was 206 nm. RESULTS The calibration curve of aucubin was in good linearity over the range of 0.053 5-1.284 μg and the regression equation was Y=539 696X+3 581.6(r=0.999 9), the average recovery was 99.75%(n=6, RSD 2.86%, fresh Rehmannia Radix) and 99.61%(n=6, RSD 2.15%, dried Rehmannia Radix) respectively; the contents of aucubin in Huai-Rehmannia Radix from different varieties and habitats were different. CONCLUSION The method has the advantage of simple operation, sensibility and stability. It can be applied to the content determination of aucubin in Huai-Rehmannia Radix.