Studies on the Determination of Stachydrine Hydrochloride in Compound Motherwort Capsules by HPLC-ELSD

OBJECTIVE To establish an HPLC method to determine the content of Stachydrine hydrochloride in compound motherwort capsules. METHODS The Agilent ZORBAX NH₂ column was used. The mobile phase was acetonitrile and water (83∶17). The flow rate was 1.0 mL·min^-1. The detector was ELSD. Logarithmic method was used to calculate the content. RESULTS The linear range of reference substance of Stachydrine hydrochloride was 5.32~15.96 μg(r=0.999); The average recovery rate was 98.66% and the relative standard deviation(RSD) was 1.9%(n=9). CONCLUSION The quantitaive method is rapid, simple, highly specific with good repeatability and accuracy.