Determination of Loureirin A in Solid Lipid Nanoparticles Encapsulating Resina Draconis by HPLC

OBJECTIVE To establish an HPLC method for determination of loureirin A content in solid lipid nanoparticles(SLN) of Resina Draconis. METHODS A DiamonsilTM C18(200 mm×4.6 mm, 5 μm) column was used for the determination of loureirin A with mobile phase of acetonitrile-1% acetic acid solution (38∶62). The flow rate was 1.3 mL·min^-1, the detection wavelength was set at 260 nm, and the column temperature was 40 ℃. RESULTS The linear range of 1oureirin A was 0.135-5.4 μg·mL^-1, Y=33.694X+1.589 8(r=0.999 9). The RSDs of within-day were 2.70%, 2.36%, 1.48%, and the RSDs of between-day were 2.70%, 2.61%, 1.97% in the high-, middle-, and low-dose Resina Draconis loaded SLN. The average recovery was 99.2%(RSD 3.9%), and the average drug encapsulation efficiency was 89.59%. CONCLUSION The method is simple and reliable for the determination of loureirin A in Resina Draconis loaded SLN.