Study on the Purification of Coumarin in Chicory with Macroporous Resin

OBJECTIVE To select the optimal purification technology of coumarin in Cichorium glafulosum Boiss. et Huet with macroporous resin. METHODS Mono factor test were employed to investigate the main parameters which may affect the purification procedures with the concentration of coumarin as index. The contents of the total coumarin and esculetin were verified by UV and HPLC separately in purified dried solid. RESULTS The optimal procedure was using D101 macroporous resin, the content of coumarin was 0.35 mg·mL^-1, the volume was 35 BV, the flow rate was 0.067 BV·min^-1. To elute with distilled water, the volume was 11 BV, the flow rate was 0.067 BV·min^-1, and with 30% ethanol, the volume was 9 BV, the flow rate was 0.033 BV·min^-1, giving up the eluate. To elute with 80% ethanol, the volume was 9 BV, the flow rate was 0.033 BV·min^-1, gathering the eluate. The content of coumarin and esculetin reached 47.00% and 6.38% separatelyin purified powders, the transfer rates of coumarin reached 81.74%. CONCLUSION The optimized technology is simple, stable, can significantly improve the contents of active constituents in Cichorium glafulosum Boiss.et Huet.