Determination of the Residual Organic Solvents in Salvianolic Acid A by GC

OBJECTIVE To establish a method for the content determination of residual organic solvents in salvianolic acid A, include alcohol and ethyl acetate. METHODS Headspace gas chromatography was adopted. Residual organic solvents were separated on HP-INNOWAX capillary column using temperature programming and nitrogen gas as carrier gas. The inlet temperature was 200 ℃. FID was used as detector with a temperature of 250 ℃. RESULTS The linear ranges of alcoho1 and ethyl acetate were 2.5-2 500 μg·mL^-1(r=0.995 3) and 0.5-2 500 μg·mL^-1(r=0.998 1), respectively, the average recoveries were 100.97% and 102.19%, respectively. RSD was both less than 5.0%. The detection limits were 0.84 mg·mL^-1 and 0.17 mg·mL^-1, respectively. The residual levels of two kinds of organic solvents in three batches of samples were in line with the standard stated in Chinese Pharmacopeia. CONCLUSION The method is simple, accurate, sensitive and reproducible. It can be used for the determination of the residua1 organic solvents in salvianolic acid A.