Determination of Stachydrine Hydrochloride in Leonuri Granule by HPLC-ELSD

OBJECTIVE To establish an HPLC-ELSD method for the determination of stachydrine hydrochloride content in Herba leonuri granule. METHODS The Venusil HILIC column(250 mm×4.6 mm, 5 μm) was adopted. The column temperature was 25 ℃. The mobile phase was acetonitrile-0.2% glacial acetic acid(80∶20). The flow rate was 0.8 mL·min^-1. The injection volume was 10 μL. The drift tube temperature was 80 ℃, and the nebulizer gas flow rate was 1 L·min^-1. RESULTS The linear range of stachydrine hydrochloride was 0.646 5-12.93 μg, r=0.999 4(n=5). The average recovery was 98.48%, RSD=1.15%(n=9). CONCLUSION The HPLC-ELSD method is convenient, rapid and accurate with good reproducibility and stability which is suitable for assay of stachydrine hydrochloride content in leonuri granule.